Topflash reporter gene
Web1 From what I can understand, TOPflash is a luciferase reporter plasmid that contains two sets of 3 copies of the wild-type TCF binding regions. If the canonical Wnt signaling is … WebCreation ORF-Firefly luciferase gene fusions. Renilla luciferase under the control of a CMV promoter is present for normalization: William Kaelin: 71248: pXPG: Firefly: Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow for increased copy number in E. coli and expression of luciferase in the cytoplasm of ...
Topflash reporter gene
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WebLuciferase reporter assays allow you to investigate promoter activity by measuring light output from luciferase enzyme that is expressed under the control of your promoter of interest. Luciferase genes vary in stability, light output and spectral characteristics, and reporter kits are available with different luciferases for both single- and dual-spectral … Web22. sep 2024 · The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level. It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously. It has broad applications across various fields of cell and molecular biology—wherever you want to …
Web21. feb 2012 · TOPflash is a TCF reporter plasmid containing two sets of three copies of wild-type TCF binding sites driven by the thymidine kinase minimal prompter and upstream of a luciferase reporter gene. WebSearch Results for Topflash Reporter Assay on Bioz, providing objective ratings for all products used in life science research. ... Bmal1 −/− organoids ( n = 3 independent …
WebThis is a CONTROL luciferase reporter with the TCF/LEF sites of Super8XTOPflash (construct M50, Addgene Plasmid #12456) being mutated. There are 6 mutated TCF/LEF … WebUnderstanding how a promoter or response element may control gene expression is easier when studied with a reporter gene. Luciferase enzymes are ideally suited for reporter …
WebThis reporter contains a firefly luciferase gene under the control of multimerized TCF/LEF responsive element located upstream of a minimal promoter. The TCF/LEF reporter is premixed with constitutively-expressing Renilla luciferase vector that serves as internal control for transfection efficiency.
WebGene/Insert name synthetic TEAD luciferase reporter Alt name YAP/TAZ luciferase reporter Alt name contains the minimal chicken TnT promoter Insert Size (bp) 360 Cloning Information Cloning method Restriction Enzyme 5′ cloning site NheI (not destroyed) 3′ cloning site NcoI (not destroyed) 5′ sequencing primer RVprimer3 humana sales representative salaryWebReagents like purified Wnt proteins, antibodies to Wnts or to b-catenin, TOPflash reporter constructs and Wnt expression constructs can be obtained from commercial sources (R&D systems, Abcam, Cell Signaling, Upstate, or Transduction Laboratories) and easily traced by … humana sales support representative salaryWebThe TCF/LEF Reporter – HEK293 Cell Line (Wnt Signaling Pathway) is designed for monitoring the activity of the Wnt/ß-catenin signaling pathway. The Wnt pathway controls a large and diverse set of cell fate decisions in embryonic development, adult organ maintenance and disease. holistic animal healing coursesWeb( N ) IGF2BP3 activates β-catenin–mediated gene transcription. The HEK293T cells were infected with either control lentivirus or IGF2BP3 lentivirus, followed by transfection with the TOPFlash reporter plasmid. TOPFlash reporter luciferase activities were measured. The relative luciferase activity (fold-change) was reported. holistic animal care online coursesWebTOPflash assay indicated transcriptional activity related to β-catenin activation. The reduction of TOPflash reporter gene activity via the exposure of AhR ligands was not seen in DLD-1, SW480,... holistic animal care tucson azWebTOPFlash reporter activity by APC-R1835G expression levels in SW480. TOPflash activity was assessed after transfection of pCMV-Neo-Bam APC-R1835G and pCMV-Neo-Bam … human ascension and the weatherWebTopflash Dual Light Reporter Gene Assay System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more holistic animal courses