2024 Phis2 snapgene

Phis2 snapgene

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WebbpHen2 (Search Vector Database) Modifications to backbone NcoI/NheI restriction sites for subsequent peptide cloning Vector type Bacterial Expression Growth in Bacteria … Webb载体介绍. pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the BD Matchmaker One-Hybrid Library Construction & Screening Kit (#K1617-1). It contains a HIS3 nutritional reporter gene, located downstream of a multiple ...

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http://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf Webb24 aug. 2024 · The promoter of each gene was cloned and inserted into the pHis2.1 vector to generate reporter constructs. TcMYC2 was fused to the GAL4 activation domain to generate the effector construct pGADT7- TcMYC2 ( Fig. 4a ). The reporter and effector constructs were co-transformed into yeast cells. how does btp differ from other police forces

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WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … WebbpYS2 (Search Vector Database) Vector type Deletion generation Growth in Bacteria Bacterial Resistance (s) Hygromycin, 200 μg/mL Growth Temperature 30°C Growth Strain (s) DH5alpha Copy number Unknown Gene/Insert Gene/Insert name gfp, hyg Species M. smegmatis Resource Information Supplemental Documents pYS2.gb Terms and Licenses WebbSee Command Line: Installation and Activation. macOS: The macOS installer is an Apple Disk Image file (.dmg). In Finder, open the disk image and drag the SnapGene application … photo booth rental 75001

SnapGene Software for everyday molecular biology

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Webb9 juni 2014 · The results showed that only the positive control and the co-transformants of pGAD-Zmhdz10 and pHIS2-cis could grow on the SD/Trp–/Leu–/His– medium containing 40 mM 3-aminotriazole (3-AT) whereas the negative control did not grow (Fig. 3B). We also constructed a pHIS2-mcis vector, and co-transformed it with the pGAD-Zmhdz10 … Webb10 aug. 2015 · The growth performance showed that the co-transformants of pGAD-SNAC3 along with pHIS2-P R1, pHIS2-P R11, or pHIS2-P R45 grew well on the SD/-Trp/-Leu/-His medium in the presence of 30mM 3-AT, whereas the growth of the co-transformants of pGAD-SNAC3 along with pHIS2-P R6 and pHIS2-P R54 was significantly inhibited, similar … photo booth rayaWebbSnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures Fast accurate construct design for all major molecular cloning … how does bt corn feed hungry nation

"WebbpHIS2 Vector Information PT3705-5 Sold as part of Catalog No. 630304 Restriction Map and Multiple Cloning Site (MCS) of pHIS2 Vector. Unique restriction sites are in bold. *Note: The Sac II site is resistant to cleavage. Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding ... " - Phis2 snapgene

Phis2 snapgene

Simulate a Restriction Digest – SnapGene Support

Webb13 feb. 2015 · Pairs of these reporter and effector plasmids, pHis2.1 and pGADT7-Rec2-StERF3 (negative control), p53His2 and pGAD-Rec2-53 (positive control) and pHis2.1-5×GCC and pGADT7-Rec2-StERF3 were introduced into yeast strain Y187 and the transformants were selected on SD medium lacking Leu and Trp. Transformed colonies … WebbOpen sequence files in SnapGene to view features, annotate, clone and use as a reference. Display enzyme sites, features, primers, ORFs, translations and more on plasmid maps or in detail on the sequence view. Add your vector directly into a cloning simulation with all restriction sites and features displayed.

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WebbpUC19 Vector. pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1). Webb23 juli 2013 · Version 2.0 - SnapGene Version 2.0 New Functionality Substantially improved alignment algorithm (better alignments, faster, supports sequences up to 1Mb) Multiple …

WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: … Webb22 sep. 2015 · Geneious is more suitable to process your NGS data (trimming, assembly,...) and then do a comparitive analysis of several samples. Cite. 3 Recommendations. 22nd Sep, 2015. Neelofar Mirza. Kumaun ...

WebbpCS2+ Sequence and Map pCS2+ Vector that allows high-level transient expression in vertebrate cells as well as in vitro transcription/translation. Sequence Author: I.M.A.G.E. … WebbSnapGene will automatically check each day and alert you if an update is available that is valid for your license. The automated checking can be turned off in Preferences - see Set …

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WebbSnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures Fast accurate construct design for all major molecular cloning … photo booth picturesWebb19 sep. 2024 · C-repeat binding factors/dehydration responsive element binding factors 1 (CBFs/DREB1s) are a small family of transcription factors that play important roles in plant resistance to various external stresses. However, functional characterization of tea plant (Camellia sinensis L.) CBF gene (CsCBF) was still seldom reported. Here, functional … how does bt wifi calling workWebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, … photo booth renoWebb6 feb. 2024 · The chloroplast comprises the outer and inner membranes that are composed of the translocon protein complexes Toc and Tic (translocon at the outer/inner envelope membrane of chloroplasts), respectively. Tic55, a chloroplast Tic protein member, was shown to be not vital for functional protein import in Arabidopsis from previous … photo booth rental akron ohioWebb26 okt. 2024 · Rapidly Simulate a Restriction Digest. To simulate a restriction digest from a DNA sequence window, select one or more enzyme sites, then click Tools → Simulate Agarose Gel. The resulting digest will appear in the next available lane in the Agarose Gel Simulation dialog. Repeat this step to populate other lanes. how does bt fibre broadband workWebbpHIS2 Plasmid description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically … how does bt whole home wifi workWebb12 apr. 2024 · 目的：筛选与启动子pHIS2-A互作的蛋白。. 方法：以启动子A构建到酵母单杂交启动子载体pHIS2，文库质粒为pGADT7-中山杉文库。. 材料：. 1．启动子克隆：pHIS2-A。. 根据实验需求进行扩增并酶切构建载体，两端的EcoRI和. SacI酶切位点，序列如下 ... photo booth rental annapolis md