Fitc dmso
WebB. Preparing FITC Dilutions Dissolve the FITC (250 mg/ bottle) in 4 mL of DMSO, by pipetting the DMSO into the container. Leave the container in a dark place at room temperature for 2 hours (or until all the FITC has dissolved.) Pour the entire dilution into a 500 mL VWR bottle. Add enough DMSO to fill the bottle to the 500 mL mark. WebFITC is soluble in DMSO and DMF. Applications. General. FITC is commonly used for labeling antibodies 2 (IgG) and also in other immunological applications. It is also often used in flow cytometry, a technology frequently used by biologists to study cellular populations with high precision 3.
Fitc dmso
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WebFITC, 1g . FITC, 100mg . Chemical name: Fluorescein isothiocyanate: Molecular weight: 389.38 . Extinction coefficient: 70,000M-1: cm-1 ... Dissolve TRITC in DMSO at 1mg/mL. 2. While stirring, slowly add 35µl of TRITC to 1mL of the 6mg/mL antibody solution and mix thoroughly. 2. Incubate for 2 hours at room temperature in the dark. WebApr 11, 2024 · 中文名称:荧光素-聚乙二醇-双棕榈酸甘油酯 英文名称:fitc-peg-dpg 外观:取决于分子量 溶解性:water, dmso, dcm 纯度:95% 活性基团:fitc 分子量:400 600 1000 2000 3400 4000 5000 10000 20000 注意事项 取用保持低温干燥,现配现用,避免频繁冻融造成产品失活 存储条件 -20 ...
WebLoudoun Benefits Office. Service Area. Ashburn, Aldie, Dulles, Leesburg, Loudoun, Purcellville, Sterling, South Riding, Loudoun County, Frederick County, Hamilton, and … WebMar 30, 2024 · 溶剂:溶于dmso、dmf,dcm,溶于水。 ... fitc-peg-nh2是一种线性异生物功能peg试剂,含有荧光素染料和胺基,与羟基甲酸、活化nhs酯、羰基(酮、醛)等反应。荧光素通常称为fitc,是生物学中常用的荧光示踪剂,用于染色细胞、组织、生物标记物或纳米 …
WebFITC (Fluorescein 5-isothiocyanate) is a fluorescence probe for the labeling of amines. FITC is a pH- and Cu2+-sensitive fluorescence dye. - Mechanism of Action & Protocol. ... Add each solvent one by one: 10% DMSO 90% …
WebThe fluorescence lifetime (τ) of the Oregon Green 514 dye in pH 9.0 buffer at 20°C is 4.2 nanoseconds. Data provided by the SPEX Fluorescence Group, Horiba Jobin Yvon Inc. The fluorescence lifetime (τ) of the Oregon Green 488 dye (Oregon Green 488 carboxylic acid, 5-isomer) in pH 9.0 buffer at 20°C is 4.1 nanoseconds. rick and morty handbagsWebAttach a 20-gauge needle to the tip of a 1 mL syringe. Use this syringe to extract the desired volume of DMSO (use 100 µL per mg of NHS-ester modification solid; depending on the modification, additional DMSO may be necessary). Use the “balloon” technique to maintain the DMSO stock bottle under nitrogen pressure and thereby keep moisture out. red sequin handbagWebAug 24, 2007 · Hello, you scientists ! I got a problem on FITC preparation . Because I'd like to add peptide-FITC mixture into bacteria and animal cell. And according to Sigma's … red sequin minnie mouse backpackWeb1. Dissolve 200 µg of FITC in 200 µl of conjugation buffer and immediately mix it with 1 ml of streptavidin solution. Note: When substituting TRITC for FITC, a similar protocol can be … red sequin dress cheapWebFidelity Mechanical Services is the premier provider of HVAC/Mechanical design, installation, and service in the Mid-Atlantic region. We provide precision end-to-end … red sequin junior bridesmaid dressesWebDMSO: 10 mg/mL (2.5mM), Need ultrasonic ( < 1 mg/ml refers to the product slightly soluble or insoluble ) 参考文献. 1. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. ... (FITC)-Dextrans to Assess Blood-Brain Barrier Disruption: Technical Considerations. Transl Stroke Res. 2011 Mar;2(1):106-11. red sequin corsetsWebExpand full name of FITC. What does FITC stand for? Is it acronym or abbreviation? FQ: FR: FRAP: FRAPPE: FROG: FRS: FS: FSA: FSD: FSI: FSS: FSTG: FTIR: FTM: … rick and morty hbomax